FIG. 2.

Viability of IBA/PCXCR4-transfected KG-1 cells (3.6 μL IBAfect and 0.6 μg pcDNAI/CXCR4) was not compromised. The number of viable cells as assessed by trypan blue exclusion A, at 12 and 24 h after CXCR4 delivery. (B) At 48 and 72 h after CXCR4 delivery and (C) optical density (OD) at 490 nm, directly proportional to the number of viable cells, was determined according to manufacturer's instructions. Results represent the mean±SEM of 3 independent experiments. *P≤0.05, statistically significant compared with untreated cells (Mann–Whitney U-test).