Table 1.
Sequence of Primers Employed for the DNA Methylation Assay
| Gene name | NANOG | SOX2 |
|---|---|---|
| Genomic regiona | EquCab2-6-35481584-35487923 | EquCab2-19-20356019-20359070 |
| Analyzed sequence | Coding sequence and 2 kb upstream size: 6344 bp | Coding sequence and 2 kb upstream size: 3053 bp |
| Primer design | Product size: 478 bp, 1.4 kb upstream of transcription start site; CpG dinucleotides included: 13 | Product size: 497 bp, 0.88 kb upstream of transcription start site; CpG dinucleotides included: 27 |
| Primerb Sequence | F:5′-TAATTTAGGGTAAGTTAGGATGGGG-3′ | F:5′-AATTTTTTTTGGAGGGAGGTTTAG-3′ |
| R:5′-AACACCTAAACTAACAACTTACCAATTC-3′ | R:5′-TATCCTACTAAAATTTCAAAAACCC-3′ |
a
UCSC genome browser on horse September 2007 (Broad/EquCab2) assembly.
b
Primers were ordered with the standard Sequenom MassCLEAVE tails (F: AGGAAGAGAG; R: CAGTAATACGACTCATAGGGAGAAGGCT).
bp, base pairs; F, forward; R, reverse.