Table 2.
Bone Marrow-Mesenchymal Stromal Cell Subpopulations in Normal and Myelodysplastic Syndrome Environments Evaluated Using Flow Cytometry Over the Course of 60 Days of Culture
| BM-MSC primary culture | BM-MSC subpopulation | Day 10 | Day 20 | Day 30 | Day 40 | Day 60 |
|---|---|---|---|---|---|---|
| NBM | STRO-1+CD73− | |||||
| % | 5.0±2.5 | 22.0±9.2 | 12.9±6.9 | 16.4±6.7 | 21.9±3.2 | |
| Cell count | 1.0±0.5 | 4.4±1.8 | 2.6±1.4 | 3.3±1.3 | 4.4±0.6 | |
| STRO-1+CD73+ | ||||||
| % | 17.0±9.9 | 25.8±6.4 | 21.7±3.3 | 24.2±5.9 | 0.7±0.2 | |
| Cell count | 3.4±2.0 | 5.2±1.3 | 4.3±0.7 | 4.8±1.2 | 0.1±0.01 | |
| STRO-1−CD73+ | ||||||
| % | 78.0±11.6 | 52.2±9.1 | 65.4±9 | 59.4±11.3 | 77.4±3.4 | |
| Cell count | 15.6±2.3 | 10.4±1.8 | 13.1±1.8 | 11.9±2.3 | 15.5±0.7 | |
| RC | STRO-1+CD73− | |||||
| % | 7.3±1.5 | 13.8±5 | 19.5±5.5 | 10.7±2.8 | 15.3±4.1 | |
| Cell count | 1.5±0.3 | 2.7±1.0 | 3.9±1.1 | 2.1±0.6 | 3.1±0.8 | |
| STRO-1+CD73+ | ||||||
| % | 20.9±2.6 | 20.4±9.8 | 27.6±5.0 | 43.0±10.2 | 39.5±5.1 | |
| Cell count | 4.2±0.5 | 4.1±2.0 | 5.5±1.0 | 8.6±2.0 | 7.9±1.0 | |
| STRO-1−CD73+ | ||||||
| % | 71.8±7.1 | 65.8±12.7 | 52.9±6.6 | 46.3±7.9 | 45.2±6.9 | |
| Cell count | 14.3±1.4 | 13.2±2.5 | 10.6±1.3 | 9.3±1.6 | 9.0±1.4 | |
| RAEB | STRO-1+CD73− | |||||
| % | 6.1±3.3 | 64.7±9.0 | 45.4±9.0 | 15.0±4.1 | 6.6±1.4 | |
| Cell count | 1.2±0.7 | 12.9±1.8 | 9.1±1.8 | 3.0±0.8 | 1.3±0.3 | |
| STRO-1+CD73+ | ||||||
| % | 18.1±1.5 | 6.8±3.2 | 14.0±5.8 | 18.0±4.1 | 24.2±7.1 | |
| Cell count | 3.6±0.3 | 1.4±0.6 | 2.8±1.2 | 3.6±0.8 | 4.8±1.4 | |
| STRO-1−CD73+ | ||||||
| % | 75.8±5.7 | 28.5±7.4 | 40.6±9.5 | 67.0±7.0 | 69.2±13.6 | |
| cell count | 15.2±1.1 | 5.7±1.5 | 8.1±1.9 | 13.4±1.4 | 13.9±2.7 | |
The values are the mean percentage and actual cell count±SD from 8 separate experiments.
Cell count=mean of actual cell count×103.
The significance level is P<0.05.
MSC, mesenchymal stromal cell; STRO-1, stromal precursor antigen-1.
The bold values highlight the main differences, in terms of distribution of the three BM-MSCs subpopulations discriminated by STRO-1 and CD73 expression, in MDS settings vs. normal controls.