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. 2011 Oct 5;21(10):1822–1830. doi: 10.1089/scd.2011.0477

FIG. 2.

FIG. 2.

In vivo identification of LRCs. (A) Composition of CFSE-positive cells after 2 weeks of tumor growth in HCT116 colon tumors, MDA231 breast tumors, and the primary breast sample 2597T tumors. (B) Representative flow cytometry plot of CFSE cell intensities for HCT116 tumor xenografts after 2 weeks of growth. White peak is the unanalyzed data according to CFSE intensity and cell number. Gray peaks represent predicted dilution populations calculated by the proliferation wizard of the Modfit software package. Six separate division peaks were calculated, with two small peaks of greatest CFSE intensity not visible. Black bar approximates the gate used during live sorting to collect the highest 5% intense cells. (C) Fluorescent staining of HCT116, MDA231, and 2597T xenograft tumors after 2 weeks of growth. Only rare cells retain a visible intensity of CFSE (white arrows) while less intense cells are still detectable by flow cytometry (scale bar=100 μm). (D) Cell cycle profiles for HCT116 xenografts. CFSE− cells (gray) and CFSE+ LRCs (black) after 2 weeks and adjacent quantification bar graphs.