Figure 2.
Growth and lysis kinetics of F. columnare G4cpN22 harboring plasmid pBV E cat by temperature induction of gene E expression. At time zero, the growth temperature of three bottles of the cultures was shifted from 26°C to 42°C (filled symbols), whereas the others served as noninduced control at 26°C (open symbols). Growth and lysis were monitored by the measurement of the OD600 (open circles and filled circles) and the determination of the number of CFU (open squares and filled squares and discontinuous lines).