Figure 2.

Bufalin induces chromatin condensation and apoptosis in A375.S2 cells. Cells were incubated with various concentrations of bufalin for 24 (a) and 48 h (b). Apoptotic cells were determined by DAPI staining and were photographed at a 200x magnification under fluorescence microscopy as described in Section 2. Each experiment was done with triple sets with similar results.