Figure 4.

Time course of the impedance (a), acidification (b) and respiration (c) of L6 rat skeletal muscle cells during 24 h incubation with NiCl₂ in the Bionas 2500 Analyzing System. Vehicle control (black), 50 mg/L (red), 25 mg/L (blue), 5 mg/L (green), 2.5 mg/L (yellow) and 0.5 mg/L (grey). Phases with running medium (RM) represent culture medium treatment without added substances. (d) Percent of control of impedance, respiration and acidification endpoint values measured after 15 h of nickel chloride exposure (n = 3). (e) Results of BrdU assay of L6 cells incubated with nickel chloride for 24 h.