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. 2012 Mar 8;12(3):3370–3393. doi: 10.3390/s120303370

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© 2012 by the authors; licensee MDPI, Basel, Switzerland

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

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Figure 7. — Time course of the impedance (a), acidification (b) and respiration (c) of L6 rat skeletal muscle cells during 24 h incubation with nicotine in the Bionas 2500 Analyzing System. Vehicle control (black), 1,000 mg/L (red), 500 mg/L (blue), 250 mg/L (green), 100 mg/L (yellow). Phases with running medium (RM) represent culture medium treatment without added substances. (d) Percent of control of impedance, respiration and acidification endpoint values measured after 15 h of nicotine exposure (n = 3). (e) Results of BrdU assay of L6 cells incubated with nicotine for 24 h.

Figure 7. — Time course of the impedance (a), acidification (b) and respiration (c) of L6 rat skeletal muscle cells during 24 h incubation with nicotine in the Bionas 2500 Analyzing System. Vehicle control (black), 1,000 mg/L (red), 500 mg/L (blue), 250 mg/L (green), 100 mg/L (yellow). Phases with running medium (RM) represent culture medium treatment without added substances. (d) Percent of control of impedance, respiration and acidification endpoint values measured after 15 h of nicotine exposure (n = 3). (e) Results of BrdU assay of L6 cells incubated with nicotine for 24 h.