Figure 3.
Lymphangiectasia and myometrial collagen accumulation are histopathological features of uteri from nulliparous, adult CMG2−/− females. M: myometrium, E: endometrium. Representative images are shown. (A) H&E stained section of a CMG2−/− uterine horn. Dilated lymphatic vessels are present throughout the myometrium, examples noted with arrowheads. There is diffuse accumulation of hyaline material and reduced myometrial cellularity in contrast with (B) H&E stained uterine horn from a CMG2+/+ littermate. (C) Higher magnification inset displaying prominent dilation of lymphatic vessels in a CMG2−/− uterus. (D, E, F) Masson’s trichrome staining of (D) wild-type and (E, F) CMG2−/− uteri highlighting the accumulation of collagen (Blue) within the myometrial layer of the CMG2−/− uterus. (G) Alcian-blue/PAS staining of a CMG2−/− uterus depicting collagen (Pink) within the myometrium.
