FIGURE 2.

Subcellular localization of PLA₂α at different leaf ages in PLA₂α-RFP transgenic Arabidopsis plants. (A,B) Epidermal cells in pre-mature leaf tissues (A) and mature leaf tissues (B) from Pro35SPLA₂α-RFP transgenic Arabidopsis plants (4-week-old) were viewed using confocal microscopy. (C) Mature leaf tissues were incubated in 1 N KNO₃ to induce plasmolysis before being viewed with confocal microscopy. The slightly decreased clarity of images in (C) appears to result from the diffusion of PLA₂α-RFP into the expanded apoplasts due to plasmolysis. White arrows in (C) indicate the plasmolyzed plasma membrane, whereas black arrows indicate extracellular spaces. Fluorescent (top), bright field (middle), and merged images (bottom) are presented. Bars = 20 μm.