FIGURE 4.

Translocation of PLA₂α to apoplasts was enhanced by the inoculation of bacteria, Pst-avrRpm1, in pre-mature young leaves. (A–C) Images showing increased fluorescence intensity and vesicle sizes followed by the translocation of PLA₂α to apoplasts at 3 h post-inoculation of Pst-avrRpm1 (C) compared to the no-treatment control (A) and 0.015% Silwet/10 mM MgCl₂-treated mock (B) in pre-mature young leaves where PLA₂α is normally localized primarily in Golgi bodies. Fluorescent (top), bright field (middle), and merged images (bottom) are presented. Bars = 20 μm.