FIGURE 5.

Enhanced translocation of PLA₂α to apoplasts in response to the inoculation of Pst-avrRpm1 in close-to-mature leaves. (A–C) Significant enhancement of PLA₂α translocation to apoplasts was observed at 3 h post-inoculation of Pst-avrRpm1 (C) compared to the no-treatment control (A) and 0.015% Silwet/10 mM MgCl₂-treated mock (B) in close-to-mature leaves where PLA₂α is already detected at a low level in the apoplasts. Fluorescent (top), bright field (middle), and merged images (bottom) are presented. Bars = 20 μm.