Table 1.
Primers used in this study. Nucleotides in bold represent mutated codons.
| primer | sequence (5′−3′) |
|---|---|
| ccPNP_pGEX6p1.fw | GCGGATCCATGTTCGATATCAAACGCAAGACG |
| ccPNP_6xHIS.fw | GCGGATCCGATGTTCGATATCAAACGCAAGACG |
| ccPNP.rev | CGGCGGCCGCTTACGCCTCTTCGGCCGCCGCTTCC |
| GWW_pGEX.fw | CCGGATCCACCGCGCCGCCCGAAAAGCCCCGTCGGGGCTGGTGGCGCCGGTAACTCGAGCG |
| GWW_pGEX.rev | CGCTCGAGTTACCGGCGCCACCAGCCCCGACGGGGCTTTTCGGGCGGCGCGGTGGATCCGG |
| GAW_pGEX.fw | CCGGATCCACCGCGCCGCCCGAAAAGCCCCGTCGGGGCGCGTGGCGCCGGTAACTCGAGCG |
| GAW_pGEX.rev | CGCTCGAGTTACCGGCGCCACGCGCCCCGACGGGGCTTTTCGGGCGGCGCGGTGGATCCGG |
| GWA_pGEX.fw | CCGGATCCACCGCGCCGCCCGAAAAGCCCCGTCGGGGCTGGGCGCGCCGGTAACTCGAGCG |
| GWA_pGEX.rev | CGCTCGAGTTACCGGCGCGCCCAGCCCCGACGGGGCTTTTCGGGCGGCGCGGTGGATCCGG |
| GAA_pGEX.fw | CCGGATCCACCGCGCCGCCCGAAAAGCCCCGTCGGGGCGCGGCGCGCCGGTAACTCGAGCG |
| GAA_pGEX.rev | CGCTCGAGTTACCGGCGCGCCGCGCCCCGACGGGGCTTTTCGGGCGGCGCGGTGGATCCGG |
| 3713_SpeI-700upcc1877-fw | AGTTACTAGTCATTGGGCAGCGCGATGAC |
| 3714_Upcc1877-3xflag-rv | CACCGTCATGGTCTTTGTAGTCCATAAAGGAAGTCTCCGCGGCG |
| 3715_Upcc1877-3xflag-fw | CGCCGCGGAGACTTCCTTTATGGACTACAAAGACCATGACGGTG |
| 3746_3xflag-cc1877-rv | CGTCGATCAGCATCTTCTTCGACATTTTATCGTCGTCATCTTTGTAGTCG |
| 3716_3xflag-cc1877-fw | CGACTACAAAGATGACGACGATAAAATGTCGAAGAAGATGCTGATCGACG |
| 3718_700cc1877-NheI-rv | TCAAGCTAGCGGTACTCATAGTCGCGCTTG |
| 3719_SpeI-700cc1877-fw | AGTTACTAGTGATGATGAAGGCGGTCGTCG |
| 3736_2664cc1877-stop-down-rv | CGGGCCGTTGTCATTCGCCCTTAGGGCGGCGCGGTGATCTCGTTCG |
| 3737_2664cc1877-stop-down-fw | CGAACGAGATCACCGCGCCGCCCTAAGGGCGAATGACAACGGCCCG |
| 3724_700downcc1877-NheI-rv | TCAAGCTAGCCCGGACTGGCCGGTGGCTTCCAGGAAGCC |