Table 2.
Crystallographic data collection and refinement.
| RNA-bound (4AM3) | Apo + GWW peptide (4AID) | Apo + GWW peptide (4AIM) | |
|---|---|---|---|
| space group | P22121 | R3 (H3 setting) | P63 |
| unit cell dimensions (Å) | a = 93.64, b = 112.06, c = 236.22 | a = 157.44, b = 157.44, c = 302.38 | a = 97.33, b = 97.33, c = 191.91 |
| crystallization conditions | 24% w/v PEG 3350, 0.1 M bis–Tris pH 5.5, 0.1 M ammonium acetate | 19% w/v PEG 3350, 0.15 M dl-malic acid | 17% w/v PEG 3000, 0.1 M trisodium citrate |
| resolution (Å) | 35.0–3.00 (3.16–3.00) | 30.0–2.60 | 40.0–3.30 (3.48–3.30) |
| light source, wavelength (Å) | diamond IO2, 0.9795 | diamond IO2, 0.9795 | diamond I24, 0.9778 |
| unique reflections | 50 090 | 41 196 | 15 391 |
| multiplicity | 4.5 (4.5) | 6.3 (6.2) | 4.7 (4.6) |
| completeness (%) | 99.2 (99.6) | 93.2 (94.8) | 99.3 (99.2) |
| intensity/σ | 9.0/2.7 | 5.5/2.4 | 7.8/2.4 |
| Rmerge (%) | 12.9 (52.3) | 21.6 (57.6) | 13.6 (65.0) |
| Wilson B factor (Å2) | 66.1 | 52.1 | 86.6 |
| refinement parameters | |||
| resolution (Å) | 35.0–3.00 | 30.0–2.60 | 40.00–3.30 |
| R-factor | 0.210 | 0.209 | 0.186 |
| R-free | 0.254 | 0.255 | 0.260 |
| number of reflections used | 47 529 | 85 978 | 15 528 |
| total number of atoms | 14 015 | 13 030 | 4957 |
| total number of amino acid residues | 1864 | 1708 | 706 |
| total number of water, phosphate | 54, 6 | 207, 3 | 2, 1 |
| total number of RNA bases | 12 | 0 | 0 |