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. 2012 May;2(5):120076. doi: 10.1098/rsob.120076

Figure 2.

Figure 2.

RhoB knockdown impairs spreading. (a) PC3 cell adhesion after 20 min and (b) spread area after 30 min on uncoated plastic, or plastic coated with Matrigel or fibronectin. Black bars denote siControl, grey bars denote siRhoB. Graphs show mean ± s.e.m. (n = 5 with >100 cells per condition; **p < 0.01). (c) Analysis of β1 and β3 integrin cell surface expression in PC3 cells. Surface proteins were biotinylated, pulled down with streptavidin beads and then analysed by immunoblotting with β1 (black bars) and β3 (grey bars) integrin antibodies and GADPH antibodies as a loading control. (i) Representative Western blot; (ii) mean levels ± s.e.m. of surface integrin levels relative to total and expressed as percentage of siControl-1 levels. n = 6. *p < 0.05, **p < 0.01.