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. Author manuscript; available in PMC: 2013 Jun 1.
Published in final edited form as: Dig Dis Sci. 2012 Apr 22;57(6):1525–1536. doi: 10.1007/s10620-012-2166-8

Figure 3.

Figure 3

mRNA expression of BMAL1, CLOCK, REVERBA, REVERBB, CRY1 and CRY2 following PER1 knockdown in Caco-2 cells. PER2 expression was measured using qPCR in Caco-2 cells stably transfected with knockdown vectors for PER1 or the scrambled sequence negative control 7 days after reaching confluence. Values are expressed as means ± SEM, normalized to actin and indexed to the negative control (n = 3 per group). Each experiment was performed a total of three times. Significance of variance between the three cell lines (SeqA, SeqB and control) was determined using ANOVA. Significant differences between each knockdown cell line and the control cell line were subsequently identified using post-hoc Tukey analysis.