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. Author manuscript; available in PMC: 2013 Jun 1.
Published in final edited form as: Dig Dis Sci. 2012 Apr 22;57(6):1525–1536. doi: 10.1007/s10620-012-2166-8

Figure 7.

Figure 7

Effect of PER1 on the SGLT1 promoter bearing E-box mutations in CHO cells. E-boxes at −306/−311, −352/−357, −827/−832 and −837/−842 on the SGLT1 promoter were mutated either singly or in combination using site-directed mutagenesis to the NheI site. CHO cells were transfected with the PER1 overexpression vector and either the wild-type SGLT1 promoter or the four E-box mutants (single or combinatorial). Firefly luciferase expression was measured at 48 hours post-transfection as a surrogate measure of SGLT1 promoter activity and normalized to Renilla luciferase to correct for variability in transfection efficiency. Results are expressed as means ± SEM, n = 3 per group, and are representative of 3 individual experiments. Significance of variance between mutants EB1-4 was determined using ANOVA. Significant differences between each mutant and the control were subsequently identified using post-hoc Tukey analysis.