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. Author manuscript; available in PMC: 2013 Jun 1.
Published in final edited form as: Dig Dis Sci. 2012 Apr 22;57(6):1525–1536. doi: 10.1007/s10620-012-2166-8

Figure 8.

Figure 8

Effect of PER1 vs pcDNA on the SGLT1 promoter bearing E-box mutations in CHO cells. The data shown in Figures 4.10 and 4.11 have been juxtaposed to allow comparison of the effects of PER1 on the wild-type and mutated SGLT1 promoter. E-boxes at −306/−311, −352/−357, −827/−832 and −837/−842 on the SGLT1 promoter were mutated either singly or in combination using site-directed mutagenesis to the NheI site. CHO cells were transfected with the PER1 overexpression vector and either the wild-type SGLT1 promoter or the four E-box mutants (single or combinatorial). Renilla luciferase was used to normalize for transfection efficiency. Firefly and Renilla luciferase activity were measured after 48 hours. All experiments were performed three times. Graphs show mean and standard error. Two-way ANOVA with Bonferroni post-tests was used to identify any interaction between the effects of PER1 and mutation of E-boxes on the SGLT1 promoter.