. Author manuscript; available in PMC: 2013 Jun 1.

Published in final edited form as: Dig Dis Sci. 2012 Apr 22;57(6):1525–1536. doi: 10.1007/s10620-012-2166-8

Table 5.

Effect of E-box mutations on SGLT1 promoter activity in CHO cells in the presence and absence of PER1 overexpression. One-way ANOVA with post-hoc Tukey was used to identify significant differences between luciferase expression of mutants and the wild-type promoter.

Expression vector	Combinatorial mutant	Fold-change relative to wild-type promoter	p-value	Interaction response	Effect
pcDNA	SGLT1-Luc mutEB1+2	2.20	<0.01	−0.267	Antagonistic
	SGLT1-Luc mutEB1+3	2.30	<0.01	−0.230	Antagonistic
	SGLT1-Luc mutEB1+2+3	2.40	<0.001	−0.381	Antagonistic
PER1	SGLT1-Luc mutEB1+2	1.23	>0.05	−0.270	Antagonistic
	SGLT1-Luc mutEB1+3	1.38	>0.05	−0.162	Antagonistic
	SGLT1-Luc mutEB1+2+3	1.51	<0.05	−0.322	Antagonistic