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. 2012 Mar;4(3):192–205. doi: 10.1002/emmm.201100199

Figure 7. Reduced progenitor marker expression in p63+/L514F and Fgfr2b−/− mice and in skin of AEC patients.

Figure 7

  1. Immunostaining reveals reduced Krt15 expression (green) in the epidermis and in the hair follicle of p63+/L514F and Fgfr2b−/− compared to +/+ control. Nuclei are stained with DAPI. Scale bar: 50 µm.
  2. Immunoblotting for Krt15 confirms decreased protein expression in p63+/L514F epidermis compared to +/+. Tubulin was used to normalize samples.
  3. Immunostaining for Krt15 (green) of skin biopsies from AEC patients (AEC1 and AEC2) and a human unaffected control (CTR) demonstrates reduced Krt15 expression in AEC skin. Scale bar: 50 µm.
  4. Real-time RT-PCR for Krt15 mRNA in human skin isolated from AEC patients or controls (CTR) reveals a quantitative reduction of Krt15 mRNA levels in the skin of AEC patients compared to wild-type. Data are represented as mean ± SD (p-value = 0.026).
  5. Immunofluorescence for Sox9 on neonatal mouse skin reveals reduced Sox9 expression in p63+/L514F and Fgfr2b−/− compared to +/+ control. Scale bar: 50 µm.
  6. Levels of Sox9 mRNA in human skin isolated from AEC patients or controls (CTR) were assessed by real-time RT-PCR. Data are represented as mean ± SD (p-value = 3.85 × 10−5).