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. 2011 Nov;3(11):637–651. doi: 10.1002/emmm.201100172

Figure 1. RORγ inhibits adipogenesis.

Figure 1

  • A. mRNA expression profile of Rorγ and Mmp3 during 3T3-L1 adipogenesis. Expression was normalized to 36b4 (n = 4).
  • B. Fluorescent staining of 3T3-L1 adipocytes overexpressing RORγ or empty vector at day 7 of differentiation with or without rosiglitazone. Automated image acquisition (blue: nuclei, red: cytosol, green: lipid droplets, 12 pictures per well, scale: 100 µm) was followed by automated image quantification using CellProfiler (n = 4).
  • C. Whole cell extracts of differentiated 3T3-L1 adipocytes were blotted against the mature adipocyte marker A-FABP using γ-Tubulin as a loading control.
  • D,E. Fluorescent staining (n = 4) and A-FABP Western blot of differentiated 3T3-L1 cells transfected with control siRNA or siRNA against RORγ 3 days before induction of differentiation. The results shown are representative of three independent differentiation experiments. Values represent means ± SD, *p ≤ 0.05, **p ≤ 0.01 and ***p ≤ 0.001.