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. 2011 Nov;3(11):637–651. doi: 10.1002/emmm.201100172

Figure 2. RORγ inhibits expression and activity of differentiation genes.

Figure 2

  • A. Western blot of nuclear extracts (RORγ, PPARγ, C/EBPα, C/EBPβ and C/EBPδ) or cytosolic extracts (MMP3, PREF-1, LPL and Adiponectin) during differentiation of 3T3-L1 cells overexpressing RORγ or empty vector. Differentiation without rosiglitazone was induced at time point 0 h. Lamin B and γ-Tubulin were used as loading controls.
  • B,C,D. qPCR for the adipogenic genes c-Jun, c-Fos and A-Fabp during differentiation of 3T3-L1 cells overexpressing RORγ or empty vector (n = 4).
  • E. Firefly luciferase activity of the PPARγ response element (PPRE) normalized to renilla luciferase before, 1 and 2 days after induction of differentiation in 3T3-L1 cells overexpressing RORγ or empty vector (n = 4).
  • F. Firefly luciferase activity of PPARγ promoter normalized to renilla luciferase in 3T3-L1 cells overexpressing RORγ or empty vector during differentiation at indicated time points (n = 8). The results shown are representative of three independent experiments. Values represent means ± SD, *p ≤ 0.05, **p ≤ 0.01 and ***p ≤ 0.001.