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. 2010 Mar;2(3):90–97. doi: 10.1002/emmm.201000061

Figure 4. ELOVL1 knockdown reduces C26:0 levels in X-ALD fibroblasts.

Figure 4

  1. ELOVL1 expression in X-ALD fibroblasts cultured for 3, 4, 5 or 6 days in medium devoid of (−) or supplemented with (+) ELOVL1 siRNA.
  2. ELOVL1 expression in X-ALD fibroblasts which were untransfected (−) or transfected (+) with siRNA targeting either cyclophilin-B (control) or ELOVL1.
  3. Effect of control siRNA (white bars) or ELOVL1 siRNA (grey bars) on cell proliferation in comparison with untransfected cells (black bars).
  4. D3-C26:0 levels in ELOVL1 siRNA transfected (grey bars) versus untransfected (black bars) X-ALD fibroblasts.
  5. C26:0 levels in control and X-ALD fibroblasts which were untransfected (black bars) and in X-ALD fibroblasts which were transfected with either control siRNA (white bars) or ELOVL1 siRNA (grey bars). Fatty acids are in nmol/mg protein. Data are mean ± SD. ***p < 0.001 by unpaired student's t-test.