Table 2.
Fatty acid levels in CHO clones expressing human ELOVL proteins
| ELOVL1 | ELOVL2 | ELOVL5 | ELOVL6 | |
|---|---|---|---|---|
| C22:5 | — | 28%** | — | — |
| C24:4 | — | >1000%*** | — | — |
| C24:5 | — | >1000%*** | — | — |
| C24:6 | — | 510%*** | — | — |
| C20:1 | 39%*** | — | 150%** | — |
| C22:1 | 56%*** | — | 129%* | — |
| C24:1 | 62%*** | — | — | — |
| C26:1 | >1000%*** | — | — | — |
| C14:0 | — | — | — | 22%*** |
| C16:0 | 164%*** | — | — | 38%*** |
| C18:0 | 147%** | — | — | 135%* |
| C24:0 | 239%*** | — | — | — |
| C26:0 | >1000%*** | — | — | — |
| C28:0 | >1000%*** | — | — | — |
Data are presented only for those transfectants for which the ANOVA p-value was <0.05. CHO cells expressing ELOVL3, ELOVL4 or ELOVL7 had the same fatty acid profiles as the mock-transfected CHO cells. For each elongase, at least three independent clones were analysed. Results are expressed as percentage relative to the amount measured in mock-transfected CHO cells. *p < 0.05, **p < 0.01 and ***p < 0.001 by ANOVA followed by Dunnett's multiple comparison test compared with mock-transfected CHO cells.