Table 3.
Fatty acid synthesis in CHO clones expressing human ELOVL proteins
| ELOVL1 | ELOVL3 | ELOVL5 | ELOVL6 | |
|---|---|---|---|---|
| D3-C18:1 | — | — | — | 145%*** |
| D3-C20:1 | 29%*** | — | 180%*** | 150%** |
| D3-C22:1 | 33%** | — | 160%** | 170%*** |
| D3-C24:1 | 35%** | — | — | — |
| D3-C26:1 | >1000%*** | — | — | — |
| D3-C18:0 | — | — | — | 170%* |
| D3-C20:0 | 200%*** | 220%*** | — | 180%*** |
| D3-C22:0 | — | 150%** | — | 170%*** |
| D3-C24:0 | 230%*** | — | — | — |
| D3-C26:0 | >1000%*** | — | — | — |
| D3-C28:0 | >1000%*** | — | — | — |
Data are presented only for those transfectants for which the ANOVA p-value was <0.05. No differences with mock-transfected CHO cells were observed for ELOVL2, ELOVL4 and ELOVL7. For each elongase, at least three independent clones were analysed. Results are expressed as percentage relative to the amount measured in mock-transfected CHO cells. *p < 0.05, **p < 0.01 and ***p < 0.001 by ANOVA followed by Dunnett's multiple comparison test compared with mock-transfected CHO cells.