Figure 6. EMCV regresses human CCRCC xenograft growth in murine dorsal skin-fold window chamber model.

1. EMCV kills HIF-overexpression tumour cells in vivo. 786-HRE-Luc cells were implanted in dorsal dermis in 6 SCID mice and tumour xenograft visualized 1 day before virus treatment (left panels). Mice were segregated into two groups of 3 where one group received intratumoural injection of live EMCV (bottom panels) while the other group received intratumoural injection of irradiated dead (d) EMCV (top panels), and tumour BLI signals visualized and measured 4 days post-treatment. Average BLI signals from each group measured in photons/seconds/area/steradian are shown with error bars representing standard errors (graph). Representative longitudinal images from the same mouse from each group are also shown (left panels).
2. EMCV kills tumour cells by necrosis. Haematoxylin and eosin (H&E) staining was performed on the resected xenografts post-termination of treatment. Representative images from each group are shown at 100× magnification. Dashed line, viable/necrotic interface; V, viable cells; N, necrotic cells; a, adipocytes; bv, blood vessels.