Figure 5. Reverse phase HPLC analysis of radiolabelled globin chains synthetized in erythroblastic culture.

HPLC profile of globin chains obtained from lysates of 15 × 10⁶ erythroid cells after 14 days of liquide culture started from normal (ND1 and ND2), thalassemic (THAL 35 = THAL-β⁰ and THAL 51 = THAL-β⁺) and thalassemic GLOBE-transduced (THAL β⁰-GLOBE or THAL β⁺-GLOBE) BM CD34⁺ cells after metabolic incorporation of radiolabelled leucine. Neo-synthetized single globin chains (α, β, δ, Aγ and Gγ) are indicated on each corresponding peak.