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. 2010 Dec;2(12):504–515. doi: 10.1002/emmm.201000103

Figure 7. Effect of MSC2032964A on LPS-induced ASK1 activation in vitro and the severity of EAE in vivo.

Figure 7

  1. Effect of MSC2032964A on LPS-induced ASK1 activation, as assessed by the phosphorylation of ASK1, p38 and JNK in cultured mouse astrocytes. Confluent astrocytes seeded in 12-well plates were pre-treated with MSC2032964A (10 µM) for 90 min and then treated with LPS (10 µg/ml) for 30 min. Cell lysates were subjected to immunoblotting with the indicated antibodies.
  2. Clinical evaluation of EAE mice treated with vehicle (n = 7) or MSC2032964A (n = 7) for a period of 40 days after MOG immunization.
  3. Representative histology of the spinal cords and optic nerves of EAE mice treated with vehicle or MSC2032964A. Lumbar spinal cords were stained with LFB and HE (upper panels) and either an anti-GFAP or anti-iba1 antibody (middle panels), and optic nerves were stained with LFB and HE (lower panels). Scale bar: 150 µm for the upper three panels and 20 µm for the lower panel.
  4. Averaged visual response amplitudes from each mouse group (n = 7) were examined by multifocal electroretinograms. *p < 0.05. Values are given in nV per square degree (nV/deg2).