Fig. 3.
Inhibition of GSK-3β inhibits Notch signaling. a The effect of pharmacological inhibition of GSK-3β with SB-216763 (25 μM for 24 h) on Notch1, Notch3 ICD and Hrt-3 protein levels in rat vSMC after 24 h. b The effect of pharmacological inhibition of GSK-3β with SB-216763 (25 μM for 24 h) on CBF-1/RBP-Jκ promoter transactivation; reversal following ectopic expression with Mut.GSK-3β and c the effect of ectopic expression of constitutively active GSK-3β (Mut.GSK-3β) on CBF-1/RBP-Jκ promoter transactivation; inhibition with 25 μM DAPT. The cumulative data represents the mean values from three independent experiments ±SEM, *p < 0.05 versus control, #p < 0.05 versus Mut.GSK-3β