Table 2. Primers and probes used in the PCR for detecting Coxiella burnetii in clinical samples and loci and primers for MLVA of C. burnetii, the Netherlands, 2007–2010*.
| Identification | Temp, °C | Primer sequence for MLVA, with label indicated, 5′ → 3′ |
|
|---|---|---|---|
| Forward | Reverse | ||
| Primers IS1111a | 60 | CATCACATTGCCGCGTTTAC | GGTTGGTCCCTCGACAACAT |
| Probe IS1111a | 60 | AATCCCCAACAACACCTCCTTATTCCCAC | |
| Probe inhibition control | 60 | ACATAATCTCTCCGACCCCACACTTCCATAC | |
| Cbu0448_ms03_12bp_7U_229bp | 60 | 6-FAM-TTGTCGATAAATCGGGAAACTT | CACTGGGAAAAGGAGAAAAAGA |
| Cbu1963_ms21_12bp_6U_210bp | 60 | NED-AGCATCTGCCTTCTCAAGTTTC | TGGGAGGTAGAAGAAAAGATGG |
| Cbu1980_ms22_11bp_6U_246bp | 60 | PET-GGGGTTTGAACATAGCAATACC | CAATATCTCTTTCTCCCGCATT |
| Cbu0259_ms24_7bp_27U_344bp | 65 | VIC-ATGAAGAAAGGATGGAGGGACT | GATAGCCTGGACAGAGGACAGT |
| Cbu0838_ms27_6bp_4U_320bp† | 65 | 6-FAM-GGGTCAGGTGGCGGGTGTG | TTCTCGCAAACGTCGCACTAACTC |
| Cbu0839_ms28_6bp_6U_480bp† | 60 | VIC-TAGAAACCGATAATCCCCTTGACA | ATTCCGCCGCCATTGAG |
| Cbu1351_ms30_18bp_6U_306bp‡ | 60 | NED-ATTTCCTCGACATCAACGTCTT | AGTCGATTTGGAAACGGATAAA |
| Cbu1418_ms31_7bp_5U_285bp‡ | 60 | PET-GGGCATCTAATCGAGATAATGG | TTTGAGAAAATTTTGGGTGCTT |
| Cbu1471_ms34_6bp_5U_210bp | 60 | 6-FAM-TGACTATCAGCGACTCGAAGAA | TCGTGCGTTAGTGTGCTTATCT |
| Cbu1941_ms36_9bp_4U_477bp‡ | 65 | VIC-GAAACCAGTCTTCCCTCAACAG | ATAACCGTCATCGTCACCTTCT |
*MLVA, multilocus variable-number tandem-repeat analyses; temp, annealing temperature. †Different primer set from the proposed set by Arricau-Bouvery et al. (11). ‡Updated after personal communication with Le Flèche, Université Paris-Sud, Orsay Cedex, France.