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. Author manuscript; available in PMC: 2012 Oct 1.
Published in final edited form as: J Neurochem. 2011 Aug 22;119(1):6–17. doi: 10.1111/j.1471-4159.2011.07397.x

Fig. 9.

Fig. 9

RNA interference-mediated suppression of Creb and c-Fos expression on Aanat, Creb and c-Fos mRNA levels. Retinal cells were transfected with 50 ng of Creb siRNA, c-Fos siRNA, or lacZ siRNA, and incubated for 24 hours before harvesting for total RNA extraction; forskolin (10μM) was added for the last 6 h. Creb, c-Fos, and Aanat levels were expressed relative to Hprt mRNA with the value of the mock-transfected control set to 1. Values are presented as mean ± SEM; n=5. A. Transfection of siRNA complementary to Creb mRNA led to a significant decrease of Creb (**p<0.001 vs. control). B. Transfection of siRNAs complementary to c-Fos mRNA or Creb mRNA significantly decreased c-Fos transcript level (#p=0.005, *p<0.05). C. Transfection of siRNAs complementary to c-Fos mRNA or Creb mRNA significantly decreased Aanat transcript level (**p<0.001). For further details see Materials and Methods.