Figure 2. Predicted targets of miR-337-3p decrease in mRNA expression on over-expression of miR-337-3p.

(A) Expression levels of miR-337-3p after 72 h transfection of NCI-H1155 cells with 50 nM miR-337-3p mimic as measured by qRT-PCR. (B) Histogram of changes in mRNA expression level as a function of miR-337-3p over-expression. The bars in black show relative frequencies of observed log₂ fold changes for all genes, while the bars in red show the distribution for genes with 3′UTRs containing at least one 7-mer corresponding to the miR-337-3p seed region. (C) The significance landscape plot for 7-mers across all genes, sorted by change in expression, from most decreased to most increased, showing 7-mers that are highly enriched in the 3′UTRs of genes that are decreased in expression. (D) Correlation between mRNA expression and motif content of 3′UTRs induced by miR-337-3p over-expression. Shown are: (1) the 7-mer motif, highlighted to show complementarity to miR-337-3p, (2) the regression coefficient, (3) the p value, (4) the number of genes containing the motif, (5) the miRNA to which the motif corresponds, with the nucleotide positions fully complementary to the highlighted sequences in column 1 shown in brackets, and the number of genes that (6) decrease in expression or (7) increase in expression by ≥2-fold. In column 6, the number of genes that contain the listed motif, but do not contain the canonical miR-337-3p seed sequence target, is given in parentheses. (E) Proportional Venn diagram showing the relationship among the genes that decrease ≥2 fold in expression and contain one or more of the five 7-mers corresponding to the mature miR-337-3p sequences.