Skip to main content
. 2012 Jun 18;7(6):e39167. doi: 10.1371/journal.pone.0039167

Figure 3. RAP1A and STAT3 are direct targets of miR-337-3p.

Figure 3

(A) miR-337-3p and its predicted interaction with target sites in RAP1A and STAT3. Shown are the structures and sequences of the miRNA:target interactions for miR-337-3p and the 3′UTRs of RAP1A and STAT3, and the predicted free energy of hybridization. The seed sequence is highlighted in red. Bases altered by site-directed mutagenesis are underlined. (B) Luciferase reporter assay. NCI-H1155 cells were co-transfected with the indicated oligos and the luciferase reporter vectors. Luciferase and β-galactosidase activities were measured after 72 h, with luciferase activity normalized to β-galactosidase activity. (C) Expression of RAP1A and STAT3 mRNA and protein levels after 72 h of transfection of NCI-H1155 cells with either 50 nM miR-337-3p, 25 nM siRNA directed against each of the genes or negative control oligos. Shown are average qRT-PCR results of three independent transfections and representative Western blot images and quantification of band intensities. (D) RAP1A and STAT3 mRNA and protein expression in NCI-H1993 cells. *, p<0.05; **, p<0.01; ***, p<0.001.