Figure 2. TLR agonists increase LRRK2 phosphorylation at Ser935.

(A & B). Primary bone marrow derived macrophages were treated with 100 ng/ml LPS for the indicated time points before cell lysis and immunoblot with indicated antibodies. (C & D) Primary bone marrow derived macrophages were treated with 1 µg/ml Pam₃CSK₄ for the indicated time points before cell lysis and immunoblot with indicated antibodies. (E) Primary bone marrow derived macrophages were treated with the following TLR agonists for 1 h. 1 µg/ml Pam₃CSK₄, 10⁸ cells HKLM, 10 µg/ml LMW and HMW Poly(I:C), 100 ng/ml LPS, 10 µg/ml Flagellin, 1 µg/ml FSL1, 1 µM CLO97 and 2.5 µM ODN1826. (F & G) Primary bone marrow derived macrophages were treated with 10 µg/ml Poly(I:C) for the indicated time points before cell lysis and immunoblot with indicated antibodies. All immunoblots are representative of at least two independent experiments.