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. 2012 Jun 18;7(6):e39042. doi: 10.1371/journal.pone.0039042

Figure 6. O. tsutsugamushi activates caspase-1 in LPS-primed BMDMs in Nlrp3-, Nlrc4- and AIM2-independent manners.

Figure 6

Wild-type (WT), MyD88-, Rip2-, Nlrp3-, Nlrc4- or Aim2-deficient BMDMs were primed with LPS (10 ng/ml) for 16 h and then treated with ATP (5 mM) for 3 h or infected with OT (ICU/cell=50) for 6 h or the indicated time periods. (A, B) IL-1β and IL-6 production from the cells was assessed by ELISA. (C) The cleaved caspase-1 and procaspase-1 were analyzed by western blotting using rabbit polyclonal antibodies specific for the p10 subunits of caspase-1. Error bars represent SD of triplicate samples. N.D.; not detected. -; untreated. ***p<0.001 versus wild-type. Data are representative of three independent experiments in A-C.