Table 3. Primers used in this study.
| No. | Name | Sequence (5′–3′) a |
| 1 | EF0031DMF EcoRI | CTGTCGAATTCCATCAGCGCTTAGGAATAATTG |
| 2 | EF0031DMR SmaI | CTGTCCCCGGGCAACATAACGTAGCCAAAGAG |
| 3 | EF0031 inside 1 | CAATAATTTAACGACTACATAGTC |
| 4 | EF0031 inside 2 | GTCACTAGTTGGCAACCAC |
| 5 | EF1027 delF | CAGCAATTGGGTTTCTTTGAA |
| 6 | EF1027 delR | TTTGATGAGATTCCGCTATGG |
| 7 | EF1027 OEL | ACTAGCGCGGCCGCTTGCTCC CCAAGTTGGTGAGTTTCCAGA |
| 8 | EF1027 OER | GGAGCAAGCGGCCGCGCTAGT AGCAATCCCAATAATCGAAGC |
| 9 | pMSP1027 BamHI | CCTGTCGGATCCGGAAATGAAGGTGTCTAAATGAA |
| 10 | pMSP1027 PstI | CCTGTCCTGCAGAATTGAGCTTCTTTTTGTTAGTC |
a
Linkers are underlined.