Figure 4. Increased chromatin association of NuRD and TIF1β and decreased histone acetylation in Carm1 knockout MEF cells.

(A) Whole cell extracts were prepared from Carm1+/+ and Carm1−/− MEFs and subjected to Western blot analysis using antibodies as indicated. (B) Chromatin fractions were prepared from Carm1+/+ and Carm1−/− MEFs and analyzed by Western blotting using antibodies as indicated. (C) Western blot analysis of core histones prepared from the wild-type and Carm1 knockout MEFs by acid extraction.