Table 2. Proteins with altered abundance in SDL (all gels, change in average ratio between 1.3 and 2, with a t-test of p<0.05).
| Spot No. | t-test | Average ratio | Identified Proteins | Accesion number (Gene ID) | Molecular mass (kD) | pI | No of peptides matched | Mascot Protein Score (>66) | Precursor ion mass | MSMS Peptide sequence | Ion score | ||
| E | T | E | T | ||||||||||
| 12 | 0.0083 | 1.74 | splicing factor 3A 677ptsubunit 1 isoform 1 | gi|5032087 (SF3A1, 10291) | ∼75 | 88.9 | ∼4.75 | 5.1 | 14 | 109 | 905.5203 | LTAQFVAR | 15 |
| 1930.8871 | FNFLNPNDPYHAYYR | 21 | |||||||||||
| 13 | 0.0055 | 1.71 | Actin, beta | gi|4501885 (ACTB, 60) | ∼42 | 42 | ∼5.6 | 5.3 | 10 | 312 | 1198.7054 | AVFPSIVGRPR | 41 |
| 1516.7026 | QEYDESGPSIVHR | 67 | |||||||||||
| 1790.8918 | SYELPDGQVITIGNER | 131 | |||||||||||
| 13 | 0.0055 | 1.71 | Actin, gamma1 | gi|4501887 (ACTG1, 71) | ∼42 | 42 | ∼5.6 | 5.3 | 10 | 312 | 1198.7054 | AVFPSIVGRPR | 41 |
| 1516.7026 | QEYDESGPSIVHR | 67 | |||||||||||
| 1790.8918 | SYELPDGQVITIGNER | 131 | |||||||||||
| 14 | 0.013 | 1.71 | lamin-A/C isoform 2 | gi|5031875 (LMNA, 4000) | ∼25 | 65 | ∼5.75 | 6.7 | 28 | 561 | 849.4828 | LAVYIDR | 34 |
| 1023.5105 | NIYSEELR | 48 | |||||||||||
| 1089.5535 | SLETENAGLR | 50 | |||||||||||
| 1182.6113 | TLEGELHDLR | 73 | |||||||||||
| 1629.8079 | LQEKEDLQELNDR | 112 | |||||||||||
| 15 | 0.018 | 1.65 | peptidyl-prolyl cis-trans isomerase FKBP4 | gi|4503729 (FKBP4, 2288) | ∼50 | 51.8 | ∼5.25 | 5.3 | 20 | 313 | 1697.8717 | RGEAHLAVNDFELAR | 56 |
| 1950.9443 | FEIGEGENLDLPYGLER | 97 | |||||||||||
| 16 | 0.0016 | 1.62 | heterogeneous nuclear 677ptribonu-cleoproteins C1/C2 isoform b | gi|117190174 (HNRPC, 3183) | ∼36 | 32.3 | ∼5.2 | 4.8 | 9 | 218 | 943.5723 | VPPPPPIAR | 56 |
| 1329.6586 | GFAFVQYVNER | 85 | |||||||||||
| 16 | 0.0016 | 1.62 | transformer-2 protein 677pthomolog beta | gi|4759098 (TRA2B, 6434) | ∼36 | 33.7 | ∼5.2 | 11.3 | 6 | 133 | 1810.897 | YGPIADVSIVYDQQSR | 91 |
| 17 | 0.0092 | 1.6 | protein CutA isoform 2 | gi|7706244 (CUTA, 51596) | ∼14 | 16.8 | ∼4.5 | 5.1 | 2 | 85 | 1533.8271 | TQSSLVPALTDFVR | 74 |
| 17 | 0.0092 | 1.6 | U6 snRNA-associated 677ptSm-like protein LSm3 | gi|7657315 (LSM3, 27258) | ∼14 | 11.8 | ∼4.5 | 4.5 | 3 | 83 | 1005.5549 | NIPMLFVR | 13 |
| 1192.7048 | GDGVVLVAPPLR | 48 | |||||||||||
| 18 | 0.021 | 1.43 | cofilin-1 | gi|5031635 (CFL1, 1072) | ∼15 | 18.5 | 5.8 | 8 | 12 | 296 | 1309.682 | AVLFCLSEDKK | 15 |
| 1337.626 | YALYDATYETK | 43 | |||||||||||
| 1790.8126 | HELQANCYEEVKDR | 76 | |||||||||||
| 2166.0964 | EILVGDVGQTVDDPYATF | 41 | |||||||||||
| 19 | 0.015 | 1.42 | cofilin-1 | gi|5031635 (CFL1, 1072) | ∼15 | 18.5 | ∼5.65 | 8.1 | 10 | 294 | 1309.682 | AVLFCLSEDKK | 45 |
| 1337.626 | YALYDATYETK | 59 | |||||||||||
| 1790.8126 | HELQANCYEEVKDR | 81 | |||||||||||
| 20 | 0.014 | 1.36 | peptidyl-prolyl cis-trans isomerase FKBP4 | gi|4503729 (FKBP4, 2288) | ∼52 | 51.8 | ∼5.3 | 5.3 | 21 | 455 | 1059.4928 | LYANMFER | 1 |
| 1103.5626 | TQLAVCQQR | 37 | |||||||||||
| 1697.8717 | RGEAHLAVNDFELAR | 98 | |||||||||||
| 1950.9443 | FEIGEGENLDLPYGLER | 143 | |||||||||||
| 21 | 0.0063 | 1.33 | actin, beta | gi|4501885 (ACTB, 60) | ∼40 | 42 | ∼5.4 | 5.3 | 13 | 187 | 1132.527 | GYSFTTTAER | 2 |
| 1198.7054 | AVFPSIVGRPR | 10 | |||||||||||
| 1516.7026 | QEYDESGPSIVHR | 29 | |||||||||||
| 1790.8918 | SYELPDGQVITIGNER | 51 | |||||||||||
| 22 | 0.0068 | 1.33 | carbonic anhydrase 1 | gi|4502517 (CA1, 759) | ∼75 | 28.9 | ∼6.5 | 6.9 | 8 | 258 | 1580.7915 | ESISVSSEQLAQFR | 105 |
| 2256.0427 | EIINVGHSFHVNFEDNDNR | 97 | |||||||||||
| 22 | 0.0068 | 1.33 | GMP synthase | gi|4504035 (GMPS, 8833) | ∼75 | 76.7 | ∼6.5 | 6.7 | 17 | 160 | 1118.6608 | VVYIFGPPVK | 20 |
| 1161.6527 | HPFPGPGLAIR | 41 | |||||||||||
| 22 | 0.0068 | 1.33 | Phosphoenolpyru-vate carboxykinase | gi|66346721 (PCK2, 5106) | ∼75 | 70.7 | ∼6.5 | 7.6 | 18 | 126 | 1118.5894 | IFHVNWFR | 17 |
Characterization of proteins fractionated by 2D-PAGE and identified by both the DeCyder software (all gels, independent t-test, change in average ratio between 1.3 and 2, with a t-test of p<0.05 ) and Mass Spectrometry (MS), ranked in order of change in average ratio. From the 11 spots picked, 13 different proteins were identified by MS. A full list of all the peptides identified for each spot is given in Table S2.