Table 5. Proteins with altered abundance in ESDL (3 gels, independent t-test, change in average ratio below <-1.3, with a t-test of p<0.05).
| Spot No. | t-test | Average ratio | Identified Proteins | Accesion number (Gene ID) | Molecular mass (kD) | pI | No of peptides matched | Mascot Protein Score (>66) | Precursor ion mass | MSMS Peptide sequence | Ion score | ||
| E | T | E | T | ||||||||||
| 36 | 0.024 | -1.71 | clathrin light chain A | gi|4502899 (CLTA, 1211) | ∼30 | 23.7 | ∼4.3 | 4.3 | 9 | 189 | 1095.5105 | ELEEWYAR | 28 |
| 1407.7267 | AIKELEEWYAR | 17 | |||||||||||
| 2352.0261 | AAEEAFVNDIDESSPGTEWER | 74 | |||||||||||
| 37 | 0.038 | -1.49 | dynactin subunit 2 | gi|5453629 (DCTN2, 10540) | ∼48 | 45 | ∼5.2 | 5 | 19 | 210 | 1598.8748 | VSALDLAVLDQVEAR | 43 |
| 1764.8398 | ENLATVEGNFASIDER | 9 | |||||||||||
| 38 | 0.00305 | -1.41 | heat shock 70 kDa protein 4 | gi|38327039 (HSPA4, 3308) | ∼100 | 94 | ∼5.1 | 5 | 29 | 269 | 1321.7111 | VLATAFDTTLGGR | 33 |
| 1495.7023 | AGGIETIANEYSDR | 5 | |||||||||||
| 1735.9265 | EFSITDVVPYPISLR | 15 | |||||||||||
| 39 | 0.004 | -1.41 | polypyrimidine tract-binding protein 1 | gi|14165466 (PTBP1, 5725) | ∼50 | 57 | ∼9 | 9.2 | 21 | 337 | 991.5432 | HQNVQLPR | 20 |
| 1431.7379 | GQPIYIQFSNHK | 34 | |||||||||||
| 2039.0959 | VTPQSLFILFGVYGDVQR | 27 | |||||||||||
| 2243.1204 | NNQFQALLQYADPVSAQHAK | 42 | |||||||||||
| 2275.2769 | IAIPGLAGAGNSVLLVSNLNPER | 35 | |||||||||||
| 40 | 0.0001 | -1.39 | eukaryotic translation initiation factor 4H | gi|11559923 (EIF4H, 7458) | ∼27 | 27.4 | ∼5.8 | 7.1 | 10 | 153 | 957.5264 | FRDGPPLR | 7 |
| 1393.6958 | EALTYDGALLGDR | 55 | |||||||||||
| 2351.2578 | TVATPLNQVANPNSAIFGGARPR | 17 | |||||||||||
| 41 | 0.024 | -1.38 | heterogeneous nuclear ribonu-cleoprotein K | gi|14165437 (HNRNPK, 3190) | ∼60 | 51 | ∼5.2 | 5.1 | 17 | 192 | 1194.6993 | NLPLPPPPPPR | 38 |
| 1780.7985 | TDYNASVSVPDSSGPER | 32 | |||||||||||
| 42 | 0.02 | -1.37 | heterogeneous nuclear ribonu-cleoproteins C1/C2 | gi|117190174 (HNRPC, 3183) | ∼37 | 32.3 | ∼5.1 | 4.8 | 13 | 240 | 943.5723 | VPPPPPIARS | 26 |
| 1316.7936 | VFIGNLNTLVVK | 10 | |||||||||||
| 1329.6586 | GFAFVQYVNER | 66 | |||||||||||
| 2117.8757 | AAEMYGSSFDLDYDFR | 34 | |||||||||||
| 43 | 0.0019 | -1.36 | HSP90 co-chaperone p23 | gi|23308579 (PTGES3, 10728) | ∼18 | 18.7 | ∼4.3 | 4.1 | 10 | 113 | 921.4644 | SILCCLR | 15 |
| 1131.5542 | KGESGQSWPR | 4 | |||||||||||
| 44 | 0.0086 | -1.35 | ATP-dependent RNA helicase DDX1 | gi|4826686 (DDX1, 1653) | ∼80 | 82.4 | ∼6.5 | 7.1 | 23 | 150 | - | - | - |
| 45 | 0.014 | -1.34 | heterogeneous nuclear ribonu-cleoproteins C1/C2 | gi|117190174 (HNRPC, 3183) | ∼37 | 32.3 | ∼5.1 | 4.8 | 12 | 276 | 943.5723 | VPPPPPIAR | 20 |
| 1316.7936 | VFIGNLNTLVVK | 11 | |||||||||||
| 1329.6586 | GFAFVQYVNER | 46 | |||||||||||
| 1698.9094 | MIAGQVLDINLAAEPK | 38 | |||||||||||
| 2117.8757 | SAAEMYGSSFDLDYDFR | 68 | |||||||||||
| 46 | 0.00096 | -1.33 | lamin-A/C | gi|5031875 (LMNA, 4000) | ∼60 | 65 | ∼5.8 | 6.7 | 25 | 199 | - | - | - |
| 47 | 0.011 | -1.32 | heterogeneous nuclear ribonu-cleoprotein K | gi|14165437 (HNRNPK, 3190) | ∼60 | 51 | ∼5.3 | 5.1 | 18 | 235 | 1053.6415 | VVLIGGKPDR | 1 |
| 1194.6993 | NLPLPPPPPPR | 49 | |||||||||||
| 1518.9365 | LLIHQSLAGGIIGVK | 34 | |||||||||||
| 1780.7985 | TDYNASVSVPDSSGPER | 16 | |||||||||||
| 48 | 0.027 | -1.32 | heterogeneous nuclear ribonu-cleoproteins C1/C2 | gi|117190174 (HNRPC, 3183) | ∼37 | 32.3 | ∼5.1 | 4.8 | 12 | 223 | 943.5723 | VPPPPPIAR | 19 |
| 1316.7936 | VFIGNLNTLVVK | 15 | |||||||||||
| 1329.6586 | GFAFVQYVNER | 50 | |||||||||||
| 1698.9094 | MIAGQVLDINLAAEPK | 20 | |||||||||||
| 2117.8757 | SAAEMYGSSFDLDYDFR | 27 | |||||||||||
| 49 | 0.027 | -1.32 | alanyl-tRNA synthetase | gi|109148542 (AARS, 16) | ∼110 | 107 | ∼5.25 | 5.3 | 21 | 155 | 1408.6743 | AVFDETYPDPVR | 33 |
| 1573.8009 | VGDQVWLFIDEPR | 5 | |||||||||||
| 1605.8635 | GGYVLHIGTIYGDLK | 3 | |||||||||||
| 50 | 0.032 | -1.32 | Heterogeneous nuclear ribonu-cleoprotein K | gi|14165437 (HNRNPK, 3190) | ∼60 | 51 | ∼5.3 | 5.1 | 16 | 209 | 1194.6993 | NLPLPPPPPPR | 50 |
| 1518.9365 | LLIHQSLAGGIIGVK | 28 | |||||||||||
| 1780.7985 | TDYNASVSVPDSSGPER | 19 | |||||||||||
| 51 | 0.0054 | -1.31 | acylamino-acid-releasing enzyme | gi|23510451 (APEH, 327) | ∼80 | 81.2 | ∼5.2 | 5.3 | 12 | 81 | 1688.8854 | QVLLSEPEEAAALYR | 30 |
| 52 | 0.0095 | -1.31 | serine/threonine-protein kinase PAK 2 | gi|32483399 (PAK2, 5062) | ∼55 | 58 | ∼5.6 | 5.8 | 14 | 102 | 2078.0601 | ECLQALEFLHANQVIHR | 15 |
| 53 | 0.0053 | -1.3 | elongation factor 1-beta | gi|4503477 (EEF1B2, 1933) | ∼28 | 24.8 | ∼4.5 | 4.3 | 8 | 108 | 945.5767 | LVPVGYGIK | 6 |
| 1603.8326 | SPAGLQVLNDYLADK | 35 | |||||||||||
| 3445.6733 | SYIEGYVPSQADVAVFEAVSSPPPADLCHALR | 7 | |||||||||||
Characterization of proteins fractionated by 2D-PAGE and identified by both the DeCyder software (3 gels, independent t-test, change in average ratio below <-1.3, with a t-test of p<0.05 ) and Mass Spectrometry (MS), ranked in order of change in average ratio. From the 18 spots picked, 14 different proteins were identified by MS. A full list of all the peptides identified for each spot is given in Table S5.