Table 6. Phosphorylated spots up-regulated in SDL.
| Spot No. | Identified Proteins | Accesion number(Gene ID) | Molecular mass (kD) | pI | No of Peptides matched | Mascot Protein Score (>66) | Precursor ion mass | MSMS Peptide sequence | Ion score | ||
| E | T | E | T | ||||||||
| 1 | Nascent poly-peptide-associated complex alpha subunit isoform b | gi|5031931 (NACA, 4666) | ∼25 | 23.4 | ∼5.1 | 4.4 | 5 | 111 | 1484.7267 | SPASDTYIVFGEAK | 19 |
| 1549.8988 | NILFVITKPDVYK | 58 | |||||||||
| 1614.8334 | IEDLSQQAQLAAAEK | 4 | |||||||||
| 2 | NDUFS3 NADH dehydrogenase (ubi-quinone) Fe-S protein 3 | gi|4758788 (NDUFS3, 4722) | ∼25 | 30.2 | ∼5.5 | 7.4 | 18 | 237 | 1295.663 | DFPLSGYVELR | 16 |
| 1366.7729 | FEIVYNLLSLR | 26 | |||||||||
| 1486.79 | VVAEPVELAQEFR | 21 | |||||||||
| 2 | HSPB1 heat shock 27 kDa protein 1 | gi|4504517 (HSPB1, 3315) | ∼25 | 22.8 | ∼5.5 | 6.2 | 9 | 146 | 1163.6207 | LFDQAFGLPR | 38 |
| 1905.9916 | LATQSNEITIPVTFESR | 37 | |||||||||
| 2 | Lamin A/C | gi|5031875 | ∼25 | 65 | ∼5.5 | 6.7 | 16 | 93 | - | - | - |
| gi|27436948 | 71 | 8.4 | |||||||||
| gi|27436946 (LMNA, 4000) | 74 | 6.8 | |||||||||
| 3 | purine nucleoside phosphorylase | gi|157168362 (PNP, 4860) | ∼29 KD | 32 | ∼6.2 | 6.7 | 16 | 253 | 1022.5781 | LVFGFLNGR | 34 |
| 1208.6344 | FEVGDIMLIR | 14 | |||||||||
| 1664.8503 | DHINLPGFSGQNPLR | 30 | |||||||||
| 2009.0127 | LTQAQIFDYGEIPNFPR | 27 | |||||||||
| 4 | heat shock protein HSP 90-alpha | gi|153792590 (HSP90AA1, 3320) | ∼36 kD | 98 | ∼4.3 | 5 | 15 | 94 | 1589.8759 | ELHINLIPNKQDR | 17 |
| 1778.9475 | HSQFIGYPITLFVEK | 5 | |||||||||
| 2015.0443 | VILHLKEDQTEYLEER | 4 | |||||||||
| 5 | heat shock protein HSP 90-alpha | gi|153792590 (HSP90AA1, 3320) | ∼34 kD | 98 | ∼4.3 | 5 | 12 | 103 | 1589.8759 | ELHINLIPNKQDR | 28 |
| 1778.9475 | HSQFIGYPITLFVEK | 17 | |||||||||
| 2015.0443 | VILHLKEDQTEYLEER | 14 | |||||||||
| 6 | heat shock protein HSP 90-beta | gi|20149594 (HSP90AB1, 3326) | ∼32 kD | 83 | ∼4.3 | 4.9 | 16 | 111 | 1194.6477 | IDIIPNPQER | 18 |
| 1564.8694 | ELKIDIIPNPQER | 3 | |||||||||
| 1808.9581 | HSQFIGYPITLYLEK | 1 | |||||||||
| 2015.0443 | VILHLKEDQTEYLEER | 4 | |||||||||
| 7 | heat shock protein HSP 90-beta | gi|20149594 (HSP90AB1, 3326) | ∼30 kD | 83 | ∼4.3 | 4.9 | 14 | 133 | 1194.6477 | IDIIPNPQER | 7 |
| 1311.5699 | EDQTEYLEER | 19 | |||||||||
| 1808.9581 | HSQFIGYPITLYLEK | 23 | |||||||||
| 2015.0443 | VILHLKEDQTEYLEER | 16 | |||||||||
| 8 | acidic leucine-rich nuclear phosphoprotein 32 family member B | gi|5454088 (ANP32B, 10541) | ∼15kD | 28.8 | ∼5 | 3.8 | 10 | 92 | 1566.8163 | LLPQLTYLDGYDR | 12 |
| 1972.9069 | SLDLFNCEVTNLNDYR | 6 | |||||||||
| 9 | 60S acidic ribosomal protein P0 | gi|4506667 (RPLP0, 6175) | ∼35 kD | 34.3 | ∼5.6 | 5.8 | 12 | 104 | 1313.71 | TSFFQALGITTK | 16 |
Characterization of proteins fractionated by 2D-PAGE, stained with Pro-Q Diamond phosphoprotein stain and identified by Image Quant v5.2 software analysis as being hyper-phosphorylated in SDL and then identified by Mass Spectrometry. From the 9 spots picked, 9 different proteins were identified by MS. A full list of all the peptides identified for each spot is given in Table S6.