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A, Intracellular stain of WT and KO B cells with isotype control (shaded histogram) or antibody against gp96N and gp96C (open histogram). B, Basophil (population I) does not express gp96C (open histogram). Shaded histograms are staining result with isotype control antibody. C, Western blot for gp96N and gp96C of sorted populations. β–actin was blotted to indicate equal loading of cell lysates.
