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. 2012 Jun 18;7(6):e39442. doi: 10.1371/journal.pone.0039442

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Liu et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A, Murine mast cells express intact gp96 without evidence for gp96MD. Left panel showed flow profile of bone marrow-derived mast cells. Right panel demonstrates gp96 immunoblot. B, Purified human basophils (left panel) only express full-length gp96 (right panel). C, Human KU812 basophil cell line does not cleave gp96 with and without ER stress induced by tunicamycin (Tu) or thapsigargin (Thap), demonstrated by gp96 immunoblot. D, Western blots for gp96 in the whole cell lysate of KU812 cells with and without ectopic expression of mMCP11.