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. 2012 Jun 18;7(6):e39497. doi: 10.1371/journal.pone.0039497

Figure 3. Role of TNFalpha, IL-1beta and MIP-1alpha in HZ-dependent induction of lysozyme release from human monocytes.

Figure 3

Cells were unfed (negative controls) or fed with HZ (positive controls) for 2 h. Afterwards, cells were incubated for 2 h alone and with a single dose (20 ng/ml) or a combination of rhTNFalpha, rhIL-1beta and rhMIP-1alpha (Panel A, mimicking approach); alternatively, cells were incubated for 2 h alone and with a single dose (30 ng/ml) or a combination of anti-hTNFalpha, anti-hIL-1beta or anti-hMIP-1alpha blocking antibodies (Panel B, blocking approach). Thereafter, lysozyme release was measured by spectrometric assay. Data are means + SEM of three independent experiments. Lysozyme release from monocytes is indicated as enzyme activity units measured in a 2 ml-well of cell supernatants. All data were evaluated for significance by ANOVA. Panel A: Vs unstimulated cells (column 1) *p<0.05, **p<0.01, ***p<0.0001; Vs untreated HZ-fed cells (column 2) °p<0.05, °°p<0.01. Panel B: Vs unstimulated cells (column 1) *p<0.0001; Vs untreated HZ-fed cells (column 2) °p<0.0001.