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. 2012 Jun 18;7(6):e39497. doi: 10.1371/journal.pone.0039497

Figure 4. Involvement of p38 MAPK pathway in HZ-dependent induction of lysozyme release from human adherent monocytes.

Figure 4

Cells were unfed (negative controls) or fed with HZ (positive controls) for 2 h; after phagocytosis, cells were incubated for 2 h alone and with 10 microM p38 MAPK synthetic inhibitor SB203580. Therefore, p38 MAPK protein expression and phosphorylation was evaluated by WB in cell lysates (Panels A and B), whereas lysozyme release was measured by spectrometric assay in cell supernatants (Panel C). Results are shown as a representative blot (A–B) or means + SEM (C) of three independent experiments. Lysozyme release from monocytes is indicated as enzyme activity units measured in a 2 ml-well of cell supernatants. In lysozyme release studies, data were also evaluated for significance by ANOVA. Vs unstimulated cells (column 1) *p<0.0001; Vs untreated HZ-fed cells (column 2) °p<0.0001.