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. 2012 Jun 18;7(6):e38927. doi: 10.1371/journal.pone.0038927

Figure 1. The number of possible crosslinked peptides increasing with sequence length.

Figure 1

The number of theoretically possible peak masses of tryptic unmodified peptides and of crosslinked peptides between 600 and 5000 Da was calculated using the software GPMAW. Data shown from left to right for a single chaperone protein here investigated, Hsp21 (UniProtKB P31170; first 44 amino acids replaced with start methionine, sequence length 184), and a chaperone model substrate protein used in ongoing crosslinking experiments MDH, (P00346, sequence length 338), and two AAA protein complexes. The AAA protein magnesium chelatase Bch IDH complex from Rhodobacter capsulatus is composed of subunit I (P26239, and C-terminal hexa-His, sequence length 356, 10 lysines), subunit D (P26175, and N-terminal hexa-His, sequence length 567, 15 lysines), and subunit H (P26162, and N-terminal hexa-His, sequence length 1195, 44 lysines). The AAA protein Clp-protease from Synechococcus sp. is composed of subunit P (Q9L4P3, and C-terminal hexa-His tag, sequence length 205, 9 lysines), subunit R (Q9L4P4, sequence length 228, 7 lysines), and subunit C (Q55023, sequence length 824, 52 lysines).