Table 1. Quantification of telomerase activity and hTERT alternative splicing variants in tumor cell lines.
Cell lines | Source | Telomerase activity*(TPG units) | hTERT overall transcripts# | hTERT alternative splicing variants | ||||||
Relative expression# | Constituent ratio★ | |||||||||
α-deletiontranscripts | β-deletiontranscripts | γ-deletiontranscripts | α-deletiontranscripts | β-deletiontranscripts | γ-deletiontranscripts | |||||
A549 | Lung adenocarcinoma | 31.43±6.47 | 17.00±2.92 | 4.25±2.14 | 2.44±0.68 | Not found | 0.25 | 0.14 | Not found | |
SPC-A1 | Lung adenocarcinoma | 14.22±9.27 | 15.14±2.70 | 3.22±0.81 | 3.97±0.81 | 0.61±0.04 | 0.21 | 0.26 | 0.04 | |
H1299 | Lung large cell carcinoma | 20.37±2.76 | 5.61±0.88 | 2.44±1.62 | 0.46±0.02 | Not found | 0.44 | 0.08 | Not found | |
PAa | Lung adenocarcinoma | 21.42±3.57 | 14.80±3.73 | 4.25±1.17 | 1.98±1.61 | Not found | 0.29 | 0.13 | Not found |
hTERT: human telomerase reverse transcriptase.
The means±standard deviation of duplicate determinations.
The means±standard deviation of triplicate determinations.
It was calculated by dividing the relative expression value of the overall transcripts by that of each deletion.