Section of multiple alignment of the Sult3a1 gene retrieved from the output result of ancestral sequences reconstruction step. From left to right, there is the species' name abbreviation, the chromosome's number, the strand in parenthesis, the position and the DNA sequence. The three dots represent parts of sequence not shown here. In bold and blue, are the exons described in Caf gene, with their number written above. The fragments of sequences that will be scanned are defined from the exon inference of the Canis gene, and are highlighted by a frame. The mis-position of nucleotides is highlighted in red. As consequence there is a frame shift which will not be detected, during the sequence scan.