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. 2012 Jun 18;7(6):e37692. doi: 10.1371/journal.pone.0037692

Figure 6. Effect of overexpression of Ssa1 on NGMC fluorescence and prion propagation in [PSI +] yeast in the presence and absence of guanidine.

Figure 6

(A) Western blot analysis of Hsp70 expression levels. The lanes are as follows: lane 1; 1074 with pRS314-GAL1-SSA1 in SD, lane 2; 1074 with pRS314-GAL1-SSA1 in SGal for 5 generations. The Pgk1 probed by anti-Pgk1 antibody on the same blot was used as an internal loading control. (B) Effect of overexpression of Ssa1 on NGMC fluorescence. Cells of strain 1074 having the empty vector pRS314 were grown in SGal for 5 generations without (a) and with 5 mM guanidine (b). Cells having pRS314-GAL1-SSA1 were grown under the same conditions for 5 generations without (c) and with 5 mM guanidine (d). (C) Effect of overexpression (o/e) of Ssa1 on [PSI +] propagation and curing by guanidine (Gdn). [PSI +] yeast containing pRS314-GAL1-SSA1 were incubated in SGal in the absence (open squares) or presence of 5 mM guanidine (solid circles). The dotted line shows the curing of [PSI +] yeast with empty vector incubated in SGal with 5 mM guanidine.