Figure 4. Cdc42-deficiency impairs HRasV12-driven tumorigenesis.

(a) Cdc42 proficient and deficient cells in a 1∶1 PBS:matrigel mixture were subcutaneously injected into the flanks of immunocompromised nude mice. Developing tumors were measured at indicated time points with calipers to determine tumor volume. n = 6. (b) 16 days post-injection mice were sacrificed and tumors excised and weighed (upper panel). A representative image of tumors from three mice are shown (lower panel). (c) Tumors were fixed, sectioned and stained with hematoxylin and eosin. (d) Left panel - The recombination status of the cdc42 locus was determined for cells prior to injection into nude mice by genomic PCR. Right panel - DNA was isolated from resulting subcutaneous tumors and the recombination status of cdc42 determined by PCR. Tumors from two representative mice were shown. The upper band corresponds to the non-recombined loxp/loxp allele from injected cells, the middle band corresponds to the wild-type cdc42 allele derived from stromal cells derived from the recipient mouse, and the lower band corresponds to the recombined cdc42 allele.