Figure 6. Growth defects observed in HRasV12-transformed cells following Cdc42 loss are partially rescued by activation of Akt signaling.

(a) Cell lysates from non-transformed and HRasV12-transformed cells were immunoblotted for phospho-Akt. Gapdh serves as a loading control. (b) Cells were infected with Ad-GFP, Ad-GFP-Cre or Ad-GFP-Cre + Ad-myr-Akt. Cell lysates were obtained and blotted for Akt expression. (c) Exponentially growing cells were pulsed with BrdU for 1 hour prior to harvest. Cells were stained with anti-BrdU and PI and analyzed by flow cytometry. (d) PI staining was used to determine the percentage of cells with 2N DNA content. Graphs represent at least three independent experiments.